|Test Name||SARS-CoV-2 (COVID-19) by NAAT/RT-PCR/TMA, Unknown Risk|
|Preferred Requirement(s)||Nasopharyngeal Swab or Oropharyngeal Swab- Viral or Universal Transport Media (VTM, UTM, UTM by Ruhof, M4RT, or VCM)- ESwab (Plastic shaft with white cap) with Swab
– 0.9% Physiological Saline
|Alternate Container(s)||Bronchoalveolar Lavage, Tracheal Aspirate, or Sputum|
|Unacceptable Specimens||Calcium alginate swabs. Swabs with wooden shafts.|
|Preferred Transport Temperature||Refrigerated: 72 Hours
*If specimen will not reach the laboratory within 72 hours of collection, freeze and ship on dry ice.
|Alternate Transport Temperature||Ambient: 72 Hours
*If specimen will not reach the laboratory within 72 hours of collection, freeze and ship on dry ice.Frozen: 1 Week
|Ordering Recommendation||For presumptive qualitative detection of nucleic acid from the 2019-Novel Coronavirus (SARS-CoV-2) in upper and lower respiratory specimens.Submission of a completed CDC COVID-19 PUI Case Report Form is strongly recommended. Specimens submitted without a completed form may be delayed.|
|Special Instructions||Submit one specimen per patient in an individually sealed bag. Clearly indicate source on container or accompanying paperwork.Nasopharyngeal Swab (recommended by CDC for initial diagnosis of COVID-19):
Insert a swab into the nostril parallel to the palate. Leave the swab in place for a few seconds to absorb secretions. Place swab immediately into UTM (viral transport media).NOTE: Use only synthetic fiber swabs with plastic shafts. Flocked swabs are acceptable. Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inactivate some viruses and inhibit PCR testing.
*SARS-CoV-2 testing can be performed on any viral transport media that has the following characteristics: maintains viability of viruses, chlamydiae, mycoplasmas and ureaplasmas; is osmotically balanced and buffered; contains Hank’s balanced salt solution with a pH indicator; uses sucrose as a preservative; contains protein and gelatin as stabilizers; contains antimicrobial agents to minimize commensal bacterial and fungus contamination. M4RT media must be kept at refrigerated or frozen temperature after collection because of limited room temperature stability of SARS-CoV-2 samples. Room temperature transport of M4RT media is only appropriate prior to sample inoculation (this is a common cause of confusion with M4RT).
Oropharyngeal Swab (e.g., throat swab):
Bronchoalveolar Lavage, Tracheal Aspirate:
|Methodology||Nucleic Acid Amplification Test (NAAT)|
|Days Performed||Sun, Mon, Tues, Wed, Thurs, Fri, Sat|